Reactivity: | M,R & H,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | MT-ND5 rabbit polyclonal antibody |
Gene Name: | NADH-ubiquinone oxidoreductase chain 5 |
Synonyms: | NADH dehydrogenase subunit 5, Mtnd5, mt-Nd5, Nd5 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MT-ND5 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. | 68 kDa |
Observed MW. | 68 kDa |
Uniprot ID: | P03921, P11661 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1:1000-1:2000 | liver, brain |
IHC/IF | Human, Rat | 1:400-1:800 | heart, kidney, lung, lung cancer |
Background
ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Images
Western blot analysis of MTND5 (GB112639) at dilution of 1: 1000 | |
Immunofluorescence of paraffin embedded rat lung using MTND5 (GB112639) at dilution of 1: 400 (400x lens) | |
Immunofluorescence of paraffin embedded human lung cancer using MTND5 (GB112639) at dilution of 1: 400 (450x lens) | |
Immunofluorescence of paraffin embedded rat heart using MTND5 (GB112639) at dilution of 1: 400 (400x lens) | |
Immunofluorescence of paraffin embedded rat kidney using MTND5 (GB112639) at dilution of 1: 400 (450x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |