Reactivity: | M,R & M |
Applications: | WB & ICC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 |
Synonyms: | B13, CI 13KD B, Complex I 13kD B, Complex I subunit B13, NDUFA5, NUFM, UQOR13 |
Immunogen: | Recombinant protein corresponding to Mouse NDUFA5 |
Uniprot ID: | Q9CPP6 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 13 kDa / 13 kDa |
Product Usage Information
WB | Mouse, Rat | 1:3000-1:5000 | heart, brain, liver |
ICC/IF | Mouse | 1: 50-1: 200 | NIH-3T3 |
Background
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Images
Western blot analysis of NDUFA5 (GB114241) at dilution of 1: 3000 | |
Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using NDUFA5(GB114241) at dilution of 1: 50-1: 200 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |