Reactivity: | M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | NECAB1 rabbit polyclonal antibody |
Gene Name: | N-terminal EF-hand calcium-binding protein 1 |
Synonyms: | EF-hand calcium-binding protein 1, Necab1, Efcbp1, NECAB1 |
Immunogen: | Recombinant protein corresponding to Mouse NECAB1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 41 kDa |
Observed MW. | 43 kDa |
Uniprot ID: | Q8BG18, Q9ESB5 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | brain, cerebral cortex, brain, cerebellum |
IHC/IF | Mouse, Rat | 1: 1000-1: 5000 | brain |
Background
This protein is the member of the EF-CBP (N-terminal EF-hand calcium binding protein) /NECAB (neuronal calcium-binding protein) family that participate in neuronal calcium signaling. EF-CBP1 (N-terminal EF-hand calcium binding protein 1), also known as STIP-1 or neuronal calcium-binding protein 1(NECAB1) is a 351 amino acid cytoplasmic protein that contains one antibiotic biosynthesis monooxygenase (ABM) domain and two EF-hand domains. Expressed in brain, EF-CBP1 interacts with copine 6 and Syntaxin, and exists as two alternatively spliced isforms.
Images
Western blot analysis of NECAB1 (GB111242) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse brain using NECAB1 (GB111242) at dilution of 1: 5000 (400x lens) | |
Immunohistochemistry of paraffin embedded rat brain using NECAB1 (GB111242) at dilution of 1: 5000 (400x lens) | |
Immunofluorescence of paraffin embedded mouse brain using NECAB1 (GB111242) at dilution of 1: 2300 (400x lens) | |
Immunofluorescence of paraffin embedded rat brain using NECAB1 (GB111242) at dilution of 1: 2300 (400x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |