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Anti-NELFe Rabbit pAb (100 μl)
| Reactivity: | H,M,R & R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Negative elongation factor E |
Synonyms: | D6S45, Negative elongation factor E, NELF E, NELFE, RD, RD RNA binding protein, RDBP, RDP |
Immunogen: | Recombinant protein corresponding to Mouse NELFe |
Uniprot ID: | P19426 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 43 kDa / 45 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 300-1: 500 | brain, testis |
IHC/IF | rat | 1: 1000-1: 2000 | testis |
Background
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (By similarity). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (By similarity).
Images
| Western blot analysis of NELFe (GB114253) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded rat testis using NELFe (GB114253) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
