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Anti-NFAT4/NF-ATc3 Rabbit pAb (100 μl)
| Reactivity: | H & H |
| Applications: | IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | NFAT4/NF-ATc3 rabbit polyclonal |
Gene Name: | Nuclear factor of activated T-cells, cytoplasmic 3 |
Synonyms: | NFATc3, NFATx, T-cell transcription factor NFAT4, NF-AT4, NF-AT4c, NFATC3 |
Immunogen: | Recombinant protein corresponding to Mouse NFAT4/NF-ATc3 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Human | 1: 500-1: 2000 | colon cancer |
IF | Human | 1: 250-1: 500 | colon cancer |
Background
NFATC3 (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells.NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids in length. It contains a calcineurin binding site (aa 109‑114), two nuclear localization sites (aa 273‑275 and 686‑688), one nuclear export site (a 1032‑1041) and an RH domain that binds DNA (aa 433‑592). There are multiple isoforms with different tissue expression profiles. Over a 900‑1035, human NFATC3 shares 93% a identity with mouse NFATC3. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa .
Images
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Immunohistochemistry analysis of paraffin-embedded human colon cancer using NFAT4/NF-ATc3 (GB111028) at dilution of1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
