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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Gamma-enolase |
Synonyms: | ENO2, HEL-S-279, NSE, Enolase 2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse NSE |
Uniprot ID: | P17183 |
Isotype: | IgG2a,κ |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 47 kDa / 47 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1:1000-1:2000 | brain, cerebral cortex, hippocampus |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | neuroendocrine tumor, brain, cerebellum |
Background
Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene.Gamma-enolase is a phosphopyruvate hydratase.Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival .
Images
| Western blot analysis of NSE (GB12376) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human neuroendocrine tumor using NSE (GB12376) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded mouse brain using NSE (GB12376) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded rat brain using NSE (GB12376) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded mouse cerebellum using NSE (GB12376) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
