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| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Nck-2 rabbit polyclonal antibody |
Gene Name: | Cytoplasmic protein NCK2 |
Synonyms: | Nck2, NCK2, GRB4, NCKbeta, NCK adaptor protein 2, Nck-2 |
Immunogen: | Recombinant protein corresponding to Mouse Nck-2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2000 | breast cancer, colon, skin |
Background
Cytoplasmic protein NCK2 (also known as NCK-beta and Grb4) is a protein encoded by the NCK2 gene. NCK belongs to family of adaptor proteins,There are two mammalian NCK genes, NCK1 and NCK2. NCK1 is located in chromosome 3 and NCK2 is located in chromosome 2. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Images
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Immunohistochemistry of paraffin embedded mouse skin using NCK (GB11900) at dilution of 1:2000 (200x lens) |
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Immunohistochemistry of paraffin embedded rat skin using NCK (GB11900) at dilution of 1:2000 (200x lens) |
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Immunofluorescence of paraffin embedded human breast cancer using NCK (GB11900) at dilution of 1:1000 (250x lens) |
 | Immunofluorescence of paraffin embedded mouse skin using NCK (GB11900) at dilution of 1:1000 (200x lens) |
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Immunofluorescence of paraffin embedded mouse colon using NCK (GB11900) at dilution of 1:1000 (250x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
