Anti-Nestin Mouse mAb (100 μl)

Nestin

Synonyms:

NES, Nbla00170, nestin

Immunogen:

Recombinant protein corresponding to Mouse Nestin

Uniprot ID:

Q6P5H2

Isotype:

IgG2a,k

Purity:

Affinity purification

Subcellular location:

Intermediate filament

IHC/IF

Mouse

1: 500-1: 1500

brain, Fetal brain, kidney

IHC analysis of Nestin(GB12160).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

IHC analysis of Nestin(GB12160).
Sample: Mouse Fetal brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

IHC analysis of Nestin(GB12160).
Sample: Mouse kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.