Anti-Nestin Rabbit pAb (100 μl)

Anti-Nestin Rabbit pAb (100 μl)

Brand: ServiceBio
Cat. #: GB115683-100
Availability: In Stock
$259.00
-+
Reactivity: H    
Applications: WB    
Host Species: Rabbit    
Clonality: Polyclonal    
Gene Name:

Nestin

Synonyms:

NES, Nbla00170, nestin

Immunogen:

Recombinant protein corresponding to Human Nestin

Uniprot ID:

P48681

Isotype:

IgG

Purity:

Affinity purification

Predicted MW. / Observed MW.

177 kDa / 250-300 kDa




Product Usage Information

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 300-1: 500

A172, SK-N-SH, U-251 MG




Background

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.




Images

01 GB115683_Nestin_C3425 08_d500_WB_WM.jpg

WB analysis of Nestin(GB115683).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.





Storage

Storage             Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


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