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Anti-Neurofilament heavy polypeptide Mouse mAb (100 μl)
| Reactivity: | M |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Neurofilament heavy polypeptide |
Synonyms: | NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, Nefh, Kiaa0845, Nfh, NF200, NF |
Immunogen: | Recombinant protein corresponding to Mouse NF200 |
Uniprot ID: | P19246 |
Isotype: | IgG2a,κ |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 117 kDa / 200 kDa |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse | 1: 1000-1: 2000 | brain, hippocampus, cerebellum |
Background
NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.
Images
 | Western blot analysis of NF200 (GB12141) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
