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Anti-Occludin Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Occludin rabbit polyclonal antibody |
Gene Name: | Occludin |
Synonyms: | OCLN, Tight junction protein occludin, PTORCH1, PPP1R115, Phosphatase 1 regulatory subunit 115, Ocln |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Occludin |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. | 59 kDa |
Observed MW. | 59 kDa |
Uniprot ID: | Q16625, Q61146, Q6P6T5 |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | colon, brain, kidney |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1500 | appendicitis, colon, colon cancer |
Background
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Under-expression of tight junction proteins is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.
Images
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WB analysis of Occludin (GB111401). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
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IHC analysis of Occludin-1 (GB111401). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 750, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of occludin1 (GB111401). Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 750, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IF analysis of occludin (GB111401). Sample: Human appendicitis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 750, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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IF analysis of occludin (GB111401). Sample: Mouse small intestine (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 750, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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IF analysis of occludin (GB111401). Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 750, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
