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Anti-PFKFB2 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 |
Synonyms: | PFKFB2, PFK-2/FBPase-2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 |
Immunogen: | Recombinant protein corresponding to Mouse PFKFB2 |
Uniprot ID: | P70265 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 60 kDa / 64 kDa |
Images
WB | Human, Mouse, Rat | 1: 500- 1: 1000 | kidney, heart |
IHC/IF | Mouse, Rat | 1: 500-1: 4000 | brain, lung |
Background
The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level. Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation . Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified. Research tudies indicate that amino acids activate PFKFB2 through Akt-dependent phosphorylation at Ser483 on PFKFB2. In addition, androgen increases the expression of PFKFB2 in prostate cancer cells.
Images
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Western blot analysis of pfkfb2 (GB11989) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin embedded rat lung using pfkfb2 (GB11989) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin embedded mouse lung using pfkfb2 (GB11989) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin embedded mouse brain using pfkfb2 (GB11989) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraffin embedded rat spleen using pfkfb2 (GB11989) at dilution of 1: 500 |
| Immunofluorescent analysis of paraffin embedded rat kidney using pfkfb2 (GB11989) at dilution of 1: 500 |
| Immunofluorescent analysis of paraffin embedded mouse lung using pfkfb2 (GB11989) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
