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Anti-PHD2 / prolyl hydroxylase Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PHD2 rabbit polyclonal |
Gene Name: | Egl nine homolog 1 |
Synonyms: | Hypoxia-inducible factor prolyl hydroxylase 2, HIF-PH2, HIF-prolyl hydroxylase 2, HPH-2, Prolyl hydroxylase domain-containing protein 2, PHD2, SM-20, Egln1 |
Immunogen: | Recombinant protein corresponding to Mouse PHD2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm. Nucleus |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Mouse, Rat | 1: 850-1: 2000 | brain, heart |
IF | Mouse, Rat | 1: 500-1: 1000 | brain, heart |
Background
Prolyl hydroxylase 2(PHD2) is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402), Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the von Hippel-Lindau ubiquitylation complex.
Images
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Immunohistochemistry analysis of paraffin-embedded mouse brain using PHD2 (GB111030) at dilution of 1: 1700 |
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Immunohistochemistry analysis of paraffin-embedded mouse heart using PHD2 (GB111030) at dilution of 1: 1700 |
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Immunohistochemistry analysis of paraffin-embedded rat brain using PHD2 (GB111030) at dilution of 1: 1700 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
