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Anti-POLR1B Rabbit pAb (100 μl)
| Reactivity: | H,M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | POLR1B rabbit polyclonal antibody |
Gene Name: | DNA-directed RNA polymerase I subunit RPA2 |
Synonyms: | RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135, Polr1b, Rpa2, Rpo1-2 |
Immunogen: | Recombinant protein corresponding to Mouse POLR1B |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 128 kDa |
Observed MW. | 128 kDa |
Uniprot ID: | Q9H9Y6, P70700 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse | 1: 1000-1: 2000 | K562, HepG2, A431, C2C12, NIH/3T3 |
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
Images
 | Western blot analysis of POLR1B (GB112646) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
