Anti-Peroxiredoxin 3/PRDX3 Rabbit pAb (100 μl)

Thioredoxin-dependent peroxide reductase, mitochondrial

Synonyms:

Antioxidant protein 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP22

Immunogen:

KLH conjugated Synthetic peptide corresponding to Mouse PRDX3

Uniprot ID:

P20108

Isotype:

lgG

Purity:

Affinity purification

Subcellular location:

Mitochondrion, Cytoplasm, Early endosome

Predicted MW. / Observed MW.

28 kDa / 26-28 KDa

WB

Mouse, Rat

1: 300-1: 500

liver, heart, brain

IHC/IF

Mouse, Rat

1: 100-1: 300

testis

WB analysis of PRDX3 (GB115503).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 300, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

WB analysis of PRDX3 (GB115503).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 300, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

IHC analysis of PRDX3 (GB115503).

Sample: Mouse testis (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0)(G1203), 98°C 20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 100, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IHC analysis of PRDX3 (GB115503).

Sample: Rat testis (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0)(G1203), 98°C 20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 100, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.