Reactivity: | H,M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | PRPF3 rabbit polyclonal antibody |
Gene Name: | U4/U6 small nuclear ribonucleoprotein Prp3 |
Synonyms: | Pre-mRNA-splicing factor 3, Prpf3 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse PRPF3 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 77 kDa |
Observed MW. | 77 kDa |
Uniprot ID: | O43395, Q922U1 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis |
IHC/IF | Mouse, Rat | 1: 1000-1: 4000 | brain, heart, kidney, testis |
Background
The PRPF3 plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).
Images
Western blot analysis of PRPF3 (GB111253) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded rat kidney using PRPF3 (GB111253) at dilution of 1: 1100 (400x lens) | |
Immunohistochemistry of paraffin embedded rat testis using PRPF3 (GB111253) at dilution of 1: 1100 (400x lens) | |
Immunofluorescence of paraffin embedded mouse heart using PRPF3 (GB111253) at dilution of 1: 2200 (400x lens) | |
Immunofluorescence of paraffin embedded rat brain using PRPF3 (GB111253) at dilution of 1: 2200 (400x lens) | |
Immunofluorescence of paraffin embedded rat kidney using PRPF3 (GB111253) at dilution of 1: 2200 (400x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |