Your shopping cart is empty!
Anti-Phospho-CREB (S133) Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Cyclic AMP-responsive element-binding protein 1 |
Synonyms: | CREB-1, CAMP-responsive element-binding protein 1, CAMP responsive element binding protein 1, Active transcription factor CREB, P-CREB |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human CREB (S133). The antibody can detect the phosphorylated form of CREB related protein ATF-1.. |
Uniprot ID: | P16220 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 37 kDa / 43 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | 293, PC-12, NIH/3T3 |
IHC/IF | Human, Mouse, Rat | 1: 700-1: 1400 | breast cancer, lung |
Background
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
Images
| Western blot analysis of Phospho-CREB (Ser133) (GB114322) at dilution of 1: 500 The figure bleow was treated with lambda phosphatase, the figure above was untreated |
| Western blot analysis of Phospho-CREB (Ser133) (GB114322) at dilution of 1: 500 The figure above was treated with non-phospho-blocking peptide, the figure below was treated with phospho- blocking peptide |
| Immunohistochemistry analysis of paraffin embedded human breast cancer using Phospho-CREB (S133) (GB114322) at dilution of 1: 700. The figure left was treated with non-phospho-blocking peptide, the figure right was treated with phospho-blocking peptide |
| Immunohistochemistry analysis of paraffin embedded mouse lung using Phospho-CREB (S133) (GB114322) at dilution of 1: 700. The figure left was untreated, the figure right was treated with lambda phosphatase |
| Immunohistochemistry analysis of paraffin embedded human breast cancer using Phospho-CREB (S133) (GB114322) at dilution of 1: 700. The figure left was untreated, the figure right was treated with lambda phosphatase |
| Immunohistochemistry analysis of paraffin embedded mouse lung using Phospho-CREB (S133) (GB114322) at dilution of 1: 700. The figure left was treated with non-phospho-blocking peptide, the figure right was treated with phospho-blocking peptide |
| Immunohistochemistry analysis of paraffin embedded rat lung using Phospho-CREB (S133) (GB114322) at dilution of 1: 700. The figure left was untreated, the figure right was treated with lambda phosphatase |
| Immunohistochemistry analysis of paraffin embedded rat lung using Phospho-CREB (S133) (GB114322) at dilution of 1: 700. The figure left was treated with non-phospho-blocking peptide, the figure right was treated with phospho-blocking peptide |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
