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Anti-Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Small ribosomal subunit protein eS6 |
Synonyms: | 40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, Phospho-RPS6 (Ser240/244), Phospho-S6 Ribosomal Protein (Ser240/244), ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human RPS6 (S240/244) |
Uniprot ID: | P62753 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 29 kDa / 32 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 2000-1: 3000 | HeLa,NIH/3T3 |
IHC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | ovarian cancer , tonsil |
Background
Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin.
Images
| WB analysis of Phospho-S6 Ribosomal Protein(Ser240/244)(GB115636). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of Phospho-S6 Ribosomal Protein(Ser240/244)(GB115636). Sample:Human ovarian cancer ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Phospho-S6 Ribosomal Protein(Ser240/244)(GB115636). Sample:Human tonsil ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Phospho-S6 Ribosomal Protein(Ser240/244)(GB115636). Sample:Mouse spleen ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Phospho-S6 Ribosomal Protein(Ser240/244)(GB115636). Sample:Rat spleen ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
