Reactivity: | H,M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Signal transducer and activator of transcription 1 |
Synonyms: | STAT1 |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human STAT1(Tyr701) |
Uniprot ID: | P42224 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 87/83 kDa / 91 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | C2C12+IFN-γ, C6 |
Background
Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2.
Images
WB analysis of Phospho-STAT1(Y701) (GB115605). | |
WB analysis of Phospho-STAT1(Y701)(GB115605). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |