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| Reactivity: | H,M,R & M,R & M |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Receptor of activated protein C kinase 1 |
Synonyms: | Rack1, RACK1, Gnb2-rs1, H12.3, HLC-7, PIG21, GNB2L1, receptor for activated C kinase 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse RACK1 |
Uniprot ID: | P68040 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 35 kDa / 35 kDa |
Images
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, 293T, MCF7 |
IHC/IF | Mouse, Rat | 1: 500-1: 2500 | brain, corpus striatum, fetal brain |
ICC/IF | Mouse | 1: 50-1: 200 | HeLa |
Background
Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Images
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Western blot analysis of Rack1 (GB11909) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded mouse brain using Rack1 (GB11909) at dilution of 1: 1200 (200x lens) |
 | Immunohistochemistry of paraffin embedded rat corpus striatum using Rack1 (GB11909) at dilution of 1: 1200 (200x lens) |
 | Immunofluorescence of paraffin embedded mouse fetal brain using Rackl (GB11909) at dilution of 1: 1000 (200x lens) |
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Immunofluorescence of paraffin embedded mouse uterus using Rackl (GB11909) at dilution of 1: 1000 (200x lens) |
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Immunofluorescence of paraffin embedded rat corpus striatum using Rackl (GB11909) at dilution of 1: 1000 (200x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using RACK1(GB11909) at dilution of 1:50-1:200 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using RACK1(GB11909) at dilution of 1:50-1:200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
