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Anti-RAP1GAP Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | RAP1GAP rabbit polyclonal antibody |
Gene Name: | Rap1 GTPase-activating protein 1 |
Synonyms: | Rap1GAP, Rap1GAP1, ARPP-90, Rap1gap, Kiaa0474, Rap1ga1 |
Immunogen: | Recombinant protein corresponding to Mouse RAP1GAP |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 73 kDa |
Observed MW. | 95 kDa |
Uniprot ID: | P47736, A2ALS5 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain |
Background
Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.
Images
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Western blot analysis of Rap1GAP (GB111473) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
