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Recombinant Anti-RFP Mouse mAb (100 μl)
| Reactivity: | |
| Applications: | ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Red fluorescent protein drFP583 |
Synonyms: | Red fluorescent protein, Red fluorescent protein eqFP611, RFPTag, RFP-Tag, RFP Tag |
Immunogen: | Recombinant protein corresponding to RFP |
Uniprot ID: | Q9U6Y8 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
ICC/IF | Species independent | 1: 500-1: 1000 | Transfected 293F cells |
Background
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions..
Images
| ICC analysis of RFP (GB155053), red. Sample: Transfected 293F cells, 4% PFA (G1101)20min. Antigen retrieval: Permeabilization Solution（G1204）, RT, 20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500,4'c overnight. Secondary antibody: 488 conjugated Goat Anti-Mouse IgG (H+L) (GB21301),1: 500,RT,20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
