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Anti-RING2 / RING1B / RNF2 Rabbit pAb (100 μl)
| Reactivity: | M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | E3 ubiquitin-protein ligase RING2 |
Synonyms: | BAP 1, BAP1, DING, HIP2 interacting protein 3, HIPI3, Protein DinG, RING finger protein 1B, RNF2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse RING2 / RING1B / RNF2 |
Uniprot ID: | Q9CQJ4 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 38 kDa / 39 kDa |
Product Usage Information
WB | Mouse | 1: 1000-1: 1500 | brain |
Background
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation . H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals . May be involved in the initiation of both imprinted and random X inactivation . Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development . PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility . E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 . Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity . Plays a role in the transcriptional repression of genes that are required for pluripotency in embryonic stem cells, thereby contributing to differentiation of the ectodermal and endodermal germ layers . Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes . Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation.
Images
| Western blot analysis of RNF2 (GB114286) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
