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| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | RNF8 rabbit polyclonal antibody |
Gene Name: | E3 ubiquitin-protein ligase RNF8 |
Synonyms: | ActA-interacting protein 37, AIP37, LaXp180, RING finger protein 8, Rnf8, RING-type E3 ubiquitin transferase RNF8 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse RNF8 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 56 kDa |
Observed MW. | 56 kDa |
Uniprot ID: | Q8VC56, Q4KLN8 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 500-1: 1000 | brain |
Background
The RNF8 (also named as KIAA0646 or E3 ubiquitin-protein ligase RNF8) contains one RING-type zinc finger and one FHA domain. RNF8 localizes in the nucleus and belongs to the RNF8 family. E2-E3 ubiquitin ligase complex is composed of the RNF8 homodimer and a E2 heterodimer of UBE2N and UBE2V2. E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.
Images
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Western blot analysis of RNF8 (GB111158) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
