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| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 40S ribosomal protein S3 |
Synonyms: | Rps3, IMR 90 ribosomal protein S3, Ribosomal protein S3 |
Immunogen: | Recombinant protein corresponding to Mouse RPS3 |
Uniprot ID: | P62908 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 27 kDa / 30 kDa |
Images
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, lung |
IHC/IF | Human, Mouse, Rat | 1: 850-1: 3000 | brain, lung, skin |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 400 | HeLa, NIH-3T3 |
Background
This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage.Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa .
Images
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Western blot analysis of RPS3 (GB111238) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human tonsil using RPS3 (GB111238) at dilution of 1: 850 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse brain using RPS3 (GB111238) at dilution of 1: 850 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse lung using RPS3 (GB111238) at dilution of 1: 850 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse spleen using RPS3 (GB111238) at dilution of 1: 850 (400x lens) |
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Immunohistochemistry of paraffin embedded rat brain using RPS3 (GB111238) at dilution of 1: 850 (400x lens) |
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Immunohistochemistry of paraffin embedded rat spleen using RPS3 (GB111238) at dilution of 1: 850 (400x lens) |
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Immunofluorescence of paraffin embedded mouse brain using RPS3 (GB111238) at dilution of 1: 1700 (400x lens) |
| Immunofluorescence of paraffin embedded mouse skin using RPS3 (GB111238) at dilution of 1: 1700 (400x lens) |
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Immunofluorescence of paraffin embedded mouse spleen using RPS3 (GB111238) at dilution of 1: 1700 (400x lens) |
| ICC analysis of RPS3(GB111238)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of RPS3(GB111238)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
