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| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | DNA repair protein RAD50 |
Synonyms: | mRad50, NBSLD, RAD502, hRad50, Mrell, hsRAD50, RAD50 homolog (S cerevisiae), RAD50 PEN, Rad50l, Truncated RAD50 protein |
Immunogen: | Recombinant protein corresponding to Mouse Rad50 |
Uniprot ID: | P70388 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 154 kDa/153 kDa |
Subcellular location: | Nucleus |
Images
WB | Mouse, Rat | 1:1000-1:3000 | spleen, lung, thymus |
IHC/IF | Mouse, Rat | 1: 250-1: 1500 | corpus striatum, lung, testis |
Background
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination.
Images
| Western blot analysis of Rad50 (GB11974) at dilution of 1: 1500 |
| Immunohistochemistry analysis of paraffin embedded mouse lung using Rad50 (GB11974) at dilution of 1: 500 |
| Immunofluorescence analysis of paraffin embedded mouse testis using Rad50 (GB11974) at dilution of 1: 500 |
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Immunohistochemistry analysis of paraffin-embedded mouse lung using Rad50 (GB11974) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded rat lung using Rad50 (GB11974) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded rat testis using Rad50 (GB11974) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
