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| Reactivity: | H & R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Replication protein A 70 kDa DNA-binding subunit |
Synonyms: | RF-A protein 1, RP-A p70, Replication factor A protein 1, Rpa1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Rpa1 |
Uniprot ID: | Q8VEE4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 69 kDa / 70 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | K562 |
IHC/IF | Rat | 1: 500-1: 1500 | spinal cord, kidney |
Background
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage.
Images
| Western blot analysis of RPA1 (GB114683) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded rat spinal cord using RPA1 (GB114683) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded rat kidney using RPA1 (GB114683) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
