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| Reactivity: | |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | S Tag |
Synonyms: | KETAAAKFERQHMDS tag, S peptide epitope tag, S peptide, S15, S-Tag, S-tag, S TAG |
Immunogen: | KLH conjugated Synthetic peptide corresponding to S-tag:KETAAAKFERQHMDS |
Isotype: | lgG |
Purity: | Affinity purification |
Product Usage Information
WB | Tag (All species) | 1: 500-1: 1000 | S-Tag fusion protein |
Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The S tag is corresponds to amino acid residues KETAAAKFERQHMDS of pancreatic ribonuclease A (RNase A). Many recombinant proteins have been engineered to express the S tag. It is believed that the peptide with its abundance of charged and polar residues could improve solubility of proteins it is attached to. Moreover, the peptide alone is thought not to fold into a distinct structure.This tag facilitates the detection, isolation, and purification of the proteins.
Images
| WB analysis of S-tag (GB121252). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
