Reactivity: | H,M,R & H,M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Monoclonal |
Gene Name: | Protein S100A4 |
Synonyms: | 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98, S100 calcium binding protein A4 |
Immunogen: | Recombinant protein corresponding to Mouse S100A4 |
Uniprot ID: | P26447, P07091, P05942 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Secreted, Nucleus |
Predicted MW. / Observed MW. | 12 kDa / 12 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 2000 | HeLa, NIH/3T3, A549, Hep G2, C2C12, PC-12, C6 |
IHC/IF | Human, Mouse, Rat | 1: 800-1: 1600 | spleen, colon, kidney, endometrial cancer |
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.
Images
Western blot analysis of S100A4 (GB15398) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded rat colon using S100A4 (GB15398) at dilution of 1: 1200 | |
Immunohistochemistry analysis of paraffin embedded mouse spleen using S100A4 (GB15398) at dilution of 1: 1200 | |
Immunohistochemistry analysis of paraffin embedded mouse colon using S100A4 (GB15398) at dilution of 1: 1200 | |
Immunohistochemistry analysis of paraffin embedded human kidney using S100A4 (GB15398) at dilution of 1: 1200 | |
Immunohistochemistry analysis of paraffin embedded human endometrial cancer using S100A4 (GB15398) at dilution of 1: 1200 | |
Immunohistochemistry analysis of paraffin embedded rat spleen using S100A4 (GB15398) at dilution of 1: 1200 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |