Your shopping cart is empty!
| Reactivity: | H,M,R & M,R |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Ribosomal protein S6 kinase beta-1 |
Synonyms: | S6K-beta-1, S6K1, P70S6K1, S6K, Ribosomal protein S6 kinase I, p70 S6 kinase alpha, Rps6kb1, 70 kDa ribosomal protein S6 kinase 1 |
Immunogen: | Recombinant protein corresponding to Mouse S6K1 |
Uniprot ID: | Q8BSK8 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 59 kDa / 70 kDa |
Images
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, testis |
ICC/IF | Mouse, Rat | 1: 100-1: 400 | NIH-3T3 |
Background
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex.
Images
|
|
Western blot analysis of P70S6K1 (GB111133) at dilution of 1: 500 |
| ICC analysis of S6K1(GB111133)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
