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Anti-SARS-CoV-2 nucleocapsid protein Mouse mAb (100 μl)
| Reactivity: | - |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | SARS-COV-2 nucleocapsid protein |
Synonyms: | 2019-nCoV nucleocapsid protein, NC,Nucleoprotein Protein N , Covid-19 |
Immunogen: | recombinant SARS-CoV-2 nucleocapsid protein expressed in E.coil. |
Isotype: | IgG2a,κ |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 50 kDa / 50 kDa |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | ----------- | 1:500-1:1000 | Use at an assay dependent sample |
Background
Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.
Images
 | Western blot analysis of N-protein (GB122133) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
