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Anti-SF3B14 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | SF3B14 rabbit polyclonal antibody |
Gene Name: | Splicing factor 3B subunit 6 |
Synonyms: | Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, Sf3b6, Sf3b14 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse SF3B14 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 14 kDa |
Observed MW. | 14 kDa |
Uniprot ID: | Q9Y3B4, P59708 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1:500-1:1000 | testis, colon |
IHC/IF | Human, Mouse, Rat | 1:500-1:2000 | brain, colon cancer, heart, liver |
Background
This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.
Images
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Western blot analysis of SF3B14 (GB111446) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human brain using SF3B14 (GB111446) at dilution of 1: 1000 (200x lens) |
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Immunohistochemistry of paraffin embedded human colon cancer using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse liver using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat heart using SF3B14 (GB111446) at dilution of 1: 1000 (300x lens) |
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Immunofluorescence of paraffin embedded mouse brain using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
