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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | SHC rabbit polyclonal antibody |
Gene Name: | SHC-transforming protein 1 |
Synonyms: | SHC1, SHC, SHCA, SHC adaptor protein 1, p46, p52, p52 SHC, p52SHC, p66, p66 SHC |
Immunogen: | Recombinant protein corresponding to Mouse SHC |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 63 kDa |
Observed MW. | 63 kDa |
Uniprot ID: | P29353, Q5M824, P98083 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, heart, brain |
Background
SHC-transforming protein 1 is a protein that in humans is encoded by the SHC1 gene. SHC has been found to be important in the regulation of apoptosis and drug resistance in mammalian cells. The gene SHC1 is located on chromosome 1 and encodes 3 main protein isoforms: p66SHC, p52SHC and p46SHC. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis.
Images
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Western blot analysis of shc (GB11566) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
