Anti-Sarcomeric Alpha Actinin Mouse mAb (100 μl)

Anti-Sarcomeric Alpha Actinin Mouse mAb (100 μl)

Brand: ServiceBio
Cat. #: GB12555-100
Availability: In Stock
$259.00
-+
Reactivity: M,R & H,M,R    
Applications: WB & IHC/IF    
Host Species: Mouse    
Clonality: Monoclonal    
Gene Name:

Alpha-actinin-2

Synonyms:

ACTN2, CMD1AA, CMH23, Actinin alpha 2, alpha Actinin 2,Actn2

Immunogen:

KLH conjugated Synthetic peptide corresponding to Mouse alpha Actinin 2

Uniprot ID:

Q9JI91

Isotype:

IgG2a,κ

Purity:

Affinity purification

Subcellular location:

Cytoplasm

Predicted MW. / Observed MW.

104 kDa / 104 kDa




Product Usage Information

Applications

Species

Dilution

Positive Sample

WB

Mouse, Rat

1: 500-1: 1000

heart, skeletal muscle

IHC/IF

Human, Mouse, Rat

1: 1500-1: 4000

brain, heart, kidney




Background

Alpha-actinin 2 is a protein which in humans is encoded by the ACTN2 gene. This gene encodes an alpha-actinin isoform that is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs. The primary function of alpha-actinin 2 is to crosslink filamentous actin molecules and titin molecules from adjoining sarcomeres at Z-discs, a function that is modulated by phospholipids. Alpha-actinin 2 also functions in docking signalling molecules at Z-discs, and additional studies have also implicated alpha-actinin 2 in the binding of cardiac ion channels, Kv1.5 in particular.




Images

Western blot analysis of α-actinin-2 (GB12555) at dilution of 1: 1000

Immunohistochemistry of paraffin embedded mouse heart using a-actinin-2 (GB12555) at dilution of 1: 2000 (200x lens)

Immunohistochemistry of paraffin embedded rat heart using a-actinin-2 (GB12555) at dilution of 1: 2000 (200x lens)
Immunohistochemistry of paraffin embedded rat skeletal muscle using a-actinin-2 (GB12555) at dilution of 1: 2000 (200x lens)
Immunofluorescence of paraffin embedded human kidney using α-actinin-2 (GB12555) at dilution of 1: 2000 (400x lens)
Immunofluorescence of paraffin embedded mouse brain using α-actinin-2 (GB12555) at dilution of 1: 2000 (400x lens)

Immunofluorescence of paraffin embedded mouse lung using α-actinin-2 (GB12555) at dilution of 1: 2000 (400x lens)

Immunofluorescence of paraffin embedded rat brain using α-actinin-2 (GB12555) at dilution of 1: 2000 (400x lens)

Immunofluorescence of paraffin embedded rat kidney using α-actinin-2 (GB12555) at dilution of 1: 2000 (400x lens)




Storage

StorageStore at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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