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| Reactivity: | H & H,M,R & H,M,R |
| Applications: | WB & IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | TPX2 rabbit polyclonal |
Gene Name: | Targeting protein for Xklp2 |
Synonyms: | Differentially expressed in cancerous and non-cancerous lung cells 2, DIL-2, p100, C20orf1, C20orf2, DIL2, HCA519, TPX2, Protein fls353 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human TPX2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 86 kDa |
Observed MW. | 100 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1000 | HeLa, HepG2, MCF7, 93T, A431 |
IHC | Human, Mouse, Rat | 1: 500-1: 1000 | lung cancer, breast cancer, prostate, testis, injured bone |
IF | Human, Mouse, Rat | 1: 300-1: 600 | lung, breast |
Background
TPX2 is essential for spindle pole and mitotic spindle formulation, playing a role in the S and G2 phases of mitosis. It is also critical for the function of protein kinase Aurora A. TPX2 autophosphorylates Aurora A, targeting it to the spindle apparatus and allowing it to function in eukaryotic meiotic and mitotic cell cycles. TPX2 has also been found to be highly expressed in cancer cells.
Images
| Western blot analysis of TPX2 (GB112703) at dilution of 1: 1000 Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate |
| Immunohistochemistry analysis of paraffin-embedded human lung cancer using TPX2 (GB112703) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human breast cancer using TPX2 (GB112703) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human prostate using TPX2 (GB112703) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse testis using TPX2 (GB112703) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded rat injured bone using TPX2 (GB112703) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse lung using TPX2 (GB112703) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat lung using TPX2 (GB112703) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat breast using TPX2 (GB112703) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
