Your shopping cart is empty!
| Reactivity: | H,M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | VCP rabbit polyclonal antibody |
Gene Name: | Transitional endoplasmic reticulum ATPase |
Synonyms: | TER ATPase, 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein, VCP |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse VCP |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 89 kDa |
Observed MW. | 97 kDa |
Uniprot ID: | P55072, Q01853 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse | 1: 500-1: 1000 | brain, lung |
Background
Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease. VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa.
Images
|
|
Western blot analysis of VCP (GB111155) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
