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Anti-Vimentin Mouse mAb (100 μl)
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Vimentin |
Synonyms: | VIM, CTRCT30, HEL113, vimentin |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse vimentin |
Uniprot ID: | P20152 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 57 kDa / 57 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | lung |
IHC/IF | Mouse, Rat | 1: 500-1: 1000 | colon, thymus, tonsil |
Background
Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
Images
| WB analysis of Vimentin (GB12192). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h. |
 | IF analysis of vimentin (GB12192). Sample: Mouse colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
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IF analysis of vimentin (GB12192). Sample: Mouse thymus (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
