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Anti-beta Actin Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Actin, cytoplasmic 1 |
Synonyms: | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse beta-Actin |
Uniprot ID: | P68369 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 42 kDa / 45 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | lung, kidney, brain |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | colon cancer, liver |
ICC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | NIH/3T3, A549, etc. |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility .
Images
| WB analysis of beta Actin (GB11001). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 5000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 5000, RT, 1h. |
| IF analysis of beta Actin (GB11001). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
