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Recombinant Anti-beta Tubulin antibody (HRP Conjugated) (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | |
| Clonality: | |
| Gene Name: | Tubulin beta chain |
Synonyms: | Tubb5, Beta 5 tubulin, Class I beta tubulin, Tubulin beta class I, TUBB1, TUBB, TUBB5, Tubulin Beta-5 Chain,β tubulin, CDCBM6 |
Immunogen: | Recombinant protein corresponding to Human beta Tubulin |
Uniprot ID: | P07437 |
Isotype: | IgG |
Conjugation | HRP |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 50 kDa / 55 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | brain, HeLa, Hep G2 |
Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-beta Tubulin antibody(ZB15140-HRP) for 3-4 hours at room temperature (20-25°C) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Images
| WB analysis of beta Tubulin(ZB15140-HRP). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-beta Tubulin antibody 1: 2000, 4°C overnight. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
