Anti-iNOS Rabbit pAb (100 μl)

Anti-iNOS Rabbit pAb (100 μl)

Brand: ServiceBio
Cat. #: GB115703-100
Availability: In Stock
$259.00
-+
Reactivity: M,R    
Applications: WB    
Host Species: Rabbit    
Clonality: Polyclonal    
Gene Name:

Nitric oxide synthase, inducible

Synonyms:

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2A, NOS2, Nitric oxide synthase 2, Hepatocyte NOS, Inducible NO synthase, Inducible NOS

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human iNOS

Uniprot ID:

P35228

Isotype:

IgG

Purity:

Affinity purification

Predicted MW. / Observed MW.

131 kDa / 131 kDa




Product Usage Information

Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1: 500-1: 1000

RAW264.7, L6




Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM.




Images

01 GB115703_iNOS_C3483(02)_d1000_WB_WM.png

WB analysis of iNOS(GB115703).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

02 GB115703_iNOS_C3483(02)_d1000_WB_WM.png

WB analysis of iNOS(GB115703).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.




Storage

Storage             Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


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