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Anti-mCherry Mouse mAb (100 μl)
| Reactivity: | |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | MCherry fluorescent protein |
Synonyms: | Monomeric cherry red fluorescent protein |
Immunogen: | Recombinant protein corresponding to mCherry |
Uniprot ID: | X5DSL3 |
Isotype: | lG2a,k |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 32 kDa / 32 kDa |
Product Usage Information
WB | Tag (All species) | 1: 500-1: 1000 | mCherry recombinant protein |
IHC/IF | Tag (All species) | 1: 500-1: 1500 | mCherry recombinant protein |
Background
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Images
| Western blot analysis of mCherry (GB122058) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
