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| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | p53 rabbit polyclonal antibody |
Gene Name: | Cellular tumor antigen p53 |
Synonyms: | TP53, BCC7, LFS1, P53, TRP53, tumor protein p53 |
Uniprot ID: | P04637 |
Immunogen: | Recombinant protein corresponding to Human p53 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Endoplasmic reticulum, Mitochondrion |
Predicted MW. | 44 kDa |
Observed MW. | 55 kDa |
Product Usage Information
WB | Human | 1: 1000-1: 2000 | HT-29, 293T, HEK293, SW480, U-251MG |
IHC/IF | Human | 1: 200-1: 1000 | cervical cancer, colon cancer |
Background
Tumor protein p53, also known as p53, cellular tumor antigen p53 , phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformation-related protein 53 (TRP53), which encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, sense cence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome.
Images
| WB analysis of P53 (GB111740). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of P53 (GB111740). Sample: Human cervical cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of P53 (GB111740). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of P53 (GB111740). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of P53 (GB111740). Sample: Human esophageal cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
