Reactivity: | M,R & H,M,R |
Applications: | WB & IHC/IF |
Host Species: | Mouse |
Clonality: | Monoclonal |
Gene Name: | Myosin-11 |
Synonyms: | Myosin heavy chain 11, Myosin heavy chain, smooth muscle isoform, SMMHC, Myh11 |
Immunogen: | Recombinant protein corresponding to Mouse Myh11 |
Uniprot ID: | O08638 |
Isotype: | IgG2a,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 227 kDa / 250 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | ovary, stomach, uterine |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 4000 | biliary duct cancer, bladder, brain |
Background
The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP.
Images
Western blot analysis of MYH11 (GB121220) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded human cancer of biliary duct using MYH11 (GB121220) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded human esophagus cancer using MYH11 (GB121220) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded mouse esophagus using MYH11 (GB121220) at dilution of 1: 1000 | |
Immunofluorescent analysis of paraffin embedded human ovarian cancer using MYH11 (GB121220) at dilution of 1: 500 | |
Immunofluorescent analysis of paraffin embedded mouse uterus using MYH11 (GB121220) at dilution of 1: 500 | |
Immunofluorescent analysis of paraffin embedded human carcinoma of urinary bladder using MYH11 (GB121220) at dilution of 1: 500 | |
Immunofluorescent analysis of paraffin embedded mouse breast using MYH11 (GB121220) at dilution of 1: 500 | |
Immunofluorescent analysis of paraffin embedded rat bladder using MYH11 (GB121220) at dilution of 1: 500 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |