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Recombinant Anti-smooth muscle Myosin heavy chain 11 Rabbit mAb (100 μl)
| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | Myosin-11 |
Synonyms: | Myosin heavy chain 11, Myosin heavy chain, smooth muscle isoform, SMMHC, Myh11 |
Immunogen: | Recombinant protein corresponding to Mouse Myh11 |
Uniprot ID: | O08638 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 227 kDa / 250 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | colon, stomach, uterine |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2000 | colon, heart |
Background
The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP.
Images
| WB analysis of smooth muscle Myosin heavy chain 11(GB15011). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of smooth muscle Myosin heavy chain 11(GB15011). Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of smooth muscle Myosin heavy chain 11(GB15011). Sample: Human smooth muscle, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of smooth muscle Myosin heavy chain 11(GB15011). Sample: Mouse colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of smooth muscle Myosin heavy chain 11(GB15011). Sample: Mouse heart, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of smooth muscle Myosin heavy chain 11(GB15011). Sample: Rat colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
