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Anti-surfactant-A Mouse mAb (100 μl)
| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Pulmonary surfactant-associated protein A1, |
Synonyms: | COLEC4, PSAP, SFTP1, SFTPA, SFTPA1B,COLEC5, PSAP, SFTP1, SFTPA, SFTPA2B |
Immunogen: | Recombinant protein corresponding to Human surfactant-A |
Uniprot ID: | Q8IWL2, Q8IWL1 |
Isotype: | IgG2b,k |
Purity: | Affinity purification |
Subcellular location: | Secreted, Cytoplasm, Cell membrane |
Predicted MW. / Observed MW. | 26 kDa /35-37 kDa (修饰化) / 26 kDa /35-37 kDa |
Product Usage Information
IHC/IF | Human | 1: 500-1: 1500 | lung |
Background
In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
Images
| IHC analysis of surfactant-A(GB12989). Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of surfactant-A(GB12989). Sample: Human lung cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of surfactant-A(GB12989). Sample: Human Lung squamous cell carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of surfactant-A(GB12989). Sample: Human lung adenocarcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
