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Anti-REDD-1/DDIT4 Rabbit pAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | DDIT4 rabbit polyclonal |
Gene Name: | DNA damage-inducible transcript 4 protein |
Synonyms: | Ddit4, DDIT4, Dig2, REDD-1, REDD1, DNA damage inducible transcript 4 |
Immunogen: | Recombinant protein corresponding to Mouse DDIT4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Mouse, Rat | 1: 2000-1: 3000 | pancreas, testis |
IF | Mouse, Rat | 1: 500-1: 1500 | pancreas, testis |
Background
DNA-damage-inducible transcript 4 (DDIT4) protein is a protein encoded by the DDIT4 gene. DDIT4 acts as a negative regulator of mTOR, a serine/threonine kinase that regulates a variety of cellular functions such as growth, proliferation and autophagy. In particular, upregulation of HIF-1 in response to hypoxia upregulates DDIT4, leading to activation of Tsc1/2 via 14–3–3 shuttling and subsequent downregulation of mTOR via Rheb. In addition to hypoxia, DDIT4 expression has also been shown to be activated by DNA damage and energy stress.
Images
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Immunohistochemistry analysis of paraffin-embedded mouse pancreas using DDIT4 (GB11937) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded rat pancreas using DDIT4 (GB11937) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded rat testis using DDIT4 (GB11937) at dilution of 1: 1500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |