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ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L) (100 μl)
| Applications: | IF/IC, FC |
| Host Species: | Donkey |
| Isotype: | ABflo™ 488 conjugated IgG |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Immunogen: | Rabbit IgG |
| Dilution: | IF/IC 1:100-1:800; FC 1:100-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 0.93 mg/mL |
| Buffer: | PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Immunofluorescence analysis of HeLa cells, using PARP1 antibody (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035) at 1:100 dilution. Blue: DAPI for nuclear staining. |
 | Flow cytometry:1X10^6 K-562 cells (negative control,left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test,orange line) or secondary antibody only (blue line).Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L)(AS035, 1:800）was used as a secondary antibody. |
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