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ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (100 μl)
| Applications: | IF/IC, FC |
| Host Species: | Goat |
| Isotype: | IgG |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Immunogen: | Mouse IgG |
| Dilution: | IF/IC 1:50-1:200; FC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 1.02 mg/mL |
| Buffer: | PBS with 0.02% sodium azide,1% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by a further incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS037, dilution 1:800) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry: 1X10^6 K-562 cells (negative control,left) and Hep G2 cells (right) were surface-stained with Mouse Anti-Human GPC3 mAb (4 μg/mL,orange line) or secondary antibody only (blue line). Non-fluorescently stained HepG2 and K-562 cells were used as blank control (red line). ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS037, 1:500)was used as a secondary antibody. |
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