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ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (100 μl)
| Applications: | IF/IC, FC |
| Host Species: | Goat |
| Isotype: | IgG |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Immunogen: | Mouse IgG |
| Dilution: | IF/IC 1:50-1:200; FC 1:100-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1.02 mg/mL |
| Buffer: | PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Confocal imaging of HeLa cells using TriMethyl-Histone H3-K9 Rabbit mAb (A22295,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:100)(Red),ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L)(AS076,dilution 1:200) (Green) |
 | Flow cytometric analysis of Positive antibody (AE005) (2.5μg/mL) in various cells (orange) compare to Rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (AS076) at 1:100. |
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