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Applications: | IF/IC, FC |
Host Species: | Goat |
Isotype: | IgG |
Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
Immunogen: | Rabbit IgG |
Dilution: | IF/IC 1:100-1:500; FC 1:100-1:500 |
Purification Method: | Affinity purification |
Concentration: | 1.02 mg/mL |
Buffer: | PBS with 0.02% sodium azide,50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual |
Images
![]() | Immunofluorescence analysis of HeLa cells, using ACO2 antibody (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L) (AS053) at 1:200 dilution. Blue: DAPI for nuclear staining. |
![]() | Flow cytometry: 1X10^6 K-562 cells (negative control,left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb (5 μl/Test,orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L)(AS053, 1:500)was used as a secondary antibody. |